The Serag method to remove protein

With 0.2 times the polysaccharide solution volume 0.04 times the volume of chloroform and butanol mixed oscillation half an hour until the separation, chloroform and water interface without precipitation so far, and to repeat the treatment 2-3 times in order to effectively remove the protein in the polysaccharides. (Aloe extract)

  Under the same conditions, pH is 5.10, polysaccharide yield was the highest. And when pH was 3.5, probably because the acid addition caused degradation of polysaccharides.

  Experiments show that, by using UF201 type ultrafiltration and reverse osmosis device can be more than 40 times of aloe gel juice concentrate.